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muffy

Goddess
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Reply with quote  #1 
Fungal extract shows anti-tumor properties in breast cancer model
By Andrew Czyzewski
27 March 2008
Br J Cancer 2008; Advance online publication

MedWire News: The medicinal mushroom Phellinus linteus is a potent inhibitor of the aggressive breast cancer cell line MDA-MB-231, and has the ability to suppress cell proliferation, invasion, and angiogenesis in vitro, US researchers have found.

Found mainly in tropical Asia, America, and Africa, P. linteus has been used in traditional oriental medicine for generations, but now scientists from the Indiana University Hospital in Indianapolis have uncovered the mechanisms employed by this fungus.

"We demonstrate that P. linteus inhibits cell proliferation as well as colony formation of highly invasive breast cancer cells through the S-phase cell cycle arrest mediated by the upregulation of expression of p27," said lead investigator Daniel Silva and colleagues in the British Journal of Cancer.

Prior experimental work in prostate and lung cancer cells grown in vitro had hinted towards the potential anti-tumor properties of P. linteus.

To investigate further, Silva and colleagues evaluated the effect of an aqueous extract of P. linteus on the behavior of MDA-MB-231 breast cancer cells in vitro.

P. linteus markedly inhibited the proliferation of MDA-MB-231 cells in a dose-dependent manner, such that at concentrations of 0.25, 0.5, and 1.0 mg/ml, cells were inhibited by 15.5%, 21.5%, and 43.1%, respectively, relative to control treatment.

Flow cytometry analysis revealed that the proportion of cells in S-phase of the cycle increased by 27%, 34%, and 44% after 1 hour, 24 hours and 48 hours of respective treatment with 0.5 mg/ml P. linteus.

Concurrent with this observation the researchers found increased expression of cyclin-dependent kinase inhibitor p27 as revealed by western blot analysis, suggesting P. linteus was arresting cells in the S-phase of their cycle.

Next Silva and colleagues demonstrated that P. linteus extract prevented migration and adhesion of MDA-MB-231 cells when cultured in an extracellular matrix with the protein vitronectin as a model for real-life invasion in mammary cells.

Finally the team found that the natural ability of cultured endothelial cells to form tubes in vitro - referred to as capillary morphogenesis - was inhibited by P. linteus extract, clearly demonstrating its anti-angiogenic properties.

The team says that this mushroom has obvious therapeutic potential but caution further studies will be needed to confirm its anti-tumor effects in vivo.


FLLoriK

Angel
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Reply with quote  #2 

I would eat mushrooms if it meant my mets would be NED! 

nosurrender

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Reply with quote  #3 
I love shrooms

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